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The 2nd reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as items (Fig. 9) - spectrophotometers. As drawn in Fig - https://papaly.com/categories/share?id=82b22f606fb5496cbfab4f86fdfbca1c. 9, the response might also involve electron transfer from H4B to the Fe, IIO2 intermediate

l9 As revealed in Fig. 9, this might be due to the fact that the H4B extreme serve as an electron sink in a later point in the reaction, so as to enable NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.